While not exhaustive, this list includes recent publications (2019 onwards) that outline the types of experimentation to which animals are subjected in Australian laboratories. More detailed profiles of Australian animal research can be found in our case studies.
21.
Sheil, ML, Chambers, M, Sharpe, B.
Topical wound anaesthesia: efficacy to mitigate piglet castration pain Journal Article
In: Aust Vet Journal, vol. 98, iss. 6, pp. 256-263, 2022, (Piglets).
Links | BibTeX | Tags: piglets
@article{nokey,
title = {Topical wound anaesthesia: efficacy to mitigate piglet castration pain},
author = {Sheil, ML, Chambers, M and Sharpe, B.},
url = {https://pubmed.ncbi.nlm.nih.gov/32096229/},
doi = {doi: 10.1111/avj.12930},
year = {2022},
date = {2022-06-01},
urldate = {2022-06-01},
journal = {Aust Vet Journal},
volume = {98},
issue = {6},
pages = {256-263},
note = {Piglets},
keywords = {piglets},
pubstate = {published},
tppubtype = {article}
}
22.
Bhedita J. Seewoo, Lauren A. Hennessy, Liz A. Jaeschke, Leah A. Mackie, Sarah J. Etherington, Sarah A. Dunlop, Paul E. Croarkin,, Jennifer Rodger
A Preclinical Study of Standard Versus Accelerated Transcranial Magnetic Stimulation for Depression in Adolescents Journal Article
In: vol. 32, no. 3, 2022, (University of Western Australia, Perron Institute for Neurological & Translational Science, WA).
Abstract | Links | BibTeX | Tags: Rats
@article{nokey,
title = {A Preclinical Study of Standard Versus Accelerated Transcranial Magnetic Stimulation for Depression in Adolescents},
author = {Bhedita J. Seewoo, Lauren A. Hennessy, Liz A. Jaeschke, Leah A. Mackie, Sarah J. Etherington, Sarah A. Dunlop, Paul E. Croarkin, and Jennifer Rodger},
doi = {10.1089/cap.2021.0100},
year = {2022},
date = {2022-04-18},
urldate = {2022-04-18},
volume = {32},
number = {3},
abstract = {Abstract
Objective: Ongoing studies are focused on adapting transcranial magnetic stimulation (TMS) for the treatment of major depressive disorder in adolescent humans. Most protocols in adolescent humans to date have delivered daily 10 Hz prefrontal stimulation with mixed results. Novel TMS dosing strategies such as accelerated TMS have recently been considered. There are knowledge gaps related to the potential clinical and pragmatic advantages of accelerated TMS. This pilot study compared the behavioral effects of a standard daily and accelerated low-intensity TMS (LI-TMS) protocol in an adolescent murine model of depression.
Methods: Male adolescent Sprague Dawley rats were placed in transparent plexiglass tubes for 2.5 hours daily for 13 days as part of a study to validate the chronic restraint stress (CRS) protocol. Rats subsequently received 10 minutes of active or sham 10 Hz LI-TMS daily for 2 weeks (standard) or three times daily for 1 week (accelerated). Behavior was assessed using the elevated plus maze and forced swim test (FST). Hippocampal neurogenesis was assessed by injection of the thymidine analogue 5-ethynyl-2′-deoxyuridine at the end of LI-TMS treatment (2 weeks standard, 1 week accelerated), followed by postmortem histological analysis.},
note = {University of Western Australia, Perron Institute for Neurological & Translational Science, WA},
keywords = {Rats},
pubstate = {published},
tppubtype = {article}
}
Abstract
Objective: Ongoing studies are focused on adapting transcranial magnetic stimulation (TMS) for the treatment of major depressive disorder in adolescent humans. Most protocols in adolescent humans to date have delivered daily 10 Hz prefrontal stimulation with mixed results. Novel TMS dosing strategies such as accelerated TMS have recently been considered. There are knowledge gaps related to the potential clinical and pragmatic advantages of accelerated TMS. This pilot study compared the behavioral effects of a standard daily and accelerated low-intensity TMS (LI-TMS) protocol in an adolescent murine model of depression.
Methods: Male adolescent Sprague Dawley rats were placed in transparent plexiglass tubes for 2.5 hours daily for 13 days as part of a study to validate the chronic restraint stress (CRS) protocol. Rats subsequently received 10 minutes of active or sham 10 Hz LI-TMS daily for 2 weeks (standard) or three times daily for 1 week (accelerated). Behavior was assessed using the elevated plus maze and forced swim test (FST). Hippocampal neurogenesis was assessed by injection of the thymidine analogue 5-ethynyl-2′-deoxyuridine at the end of LI-TMS treatment (2 weeks standard, 1 week accelerated), followed by postmortem histological analysis.
Objective: Ongoing studies are focused on adapting transcranial magnetic stimulation (TMS) for the treatment of major depressive disorder in adolescent humans. Most protocols in adolescent humans to date have delivered daily 10 Hz prefrontal stimulation with mixed results. Novel TMS dosing strategies such as accelerated TMS have recently been considered. There are knowledge gaps related to the potential clinical and pragmatic advantages of accelerated TMS. This pilot study compared the behavioral effects of a standard daily and accelerated low-intensity TMS (LI-TMS) protocol in an adolescent murine model of depression.
Methods: Male adolescent Sprague Dawley rats were placed in transparent plexiglass tubes for 2.5 hours daily for 13 days as part of a study to validate the chronic restraint stress (CRS) protocol. Rats subsequently received 10 minutes of active or sham 10 Hz LI-TMS daily for 2 weeks (standard) or three times daily for 1 week (accelerated). Behavior was assessed using the elevated plus maze and forced swim test (FST). Hippocampal neurogenesis was assessed by injection of the thymidine analogue 5-ethynyl-2′-deoxyuridine at the end of LI-TMS treatment (2 weeks standard, 1 week accelerated), followed by postmortem histological analysis.
23.
Mawj Mandwie, Jordan A Piper, Catherine A Gorrie, Kevin A Keay, Giuseppe Musumeci , Ghaith Al-Badri, Alessandro Castorina
Rapid GFAP and Iba1 expression changes in the female rat brain following spinal cord injury Journal Article
In: 2022, (University of Technology Sydney).
@article{nokey,
title = {Rapid GFAP and Iba1 expression changes in the female rat brain following spinal cord injury},
author = {Mawj Mandwie, Jordan A Piper, Catherine A Gorrie, Kevin A Keay, Giuseppe Musumeci , Ghaith Al-Badri, Alessandro Castorina},
url = {https://pubmed.ncbi.nlm.nih.gov/34269213/},
year = {2022},
date = {2022-02-17},
urldate = {2022-02-17},
note = {University of Technology Sydney},
keywords = {Rats},
pubstate = {published},
tppubtype = {article}
}
24.
Nafiseh Atapour, Katrina H Worthy, Marcello G P Rosa
Remodeling of lateral geniculate nucleus projections to extrastriate area MT following long-term lesions of striate cortex Journal Article
In: 2022, (Monash University).
Abstract | Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Remodeling of lateral geniculate nucleus projections to extrastriate area MT following long-term lesions of striate cortex},
author = {Nafiseh Atapour, Katrina H Worthy, Marcello G P Rosa},
url = {https://pubmed.ncbi.nlm.nih.gov/35058366/},
doi = {10.1073/pnas.2117137119},
year = {2022},
date = {2022-01-25},
urldate = {2022-01-25},
abstract = {Here, we report on a previously unknown form of thalamocortical plasticity observed following lesions of the primary visual area (V1) in marmoset monkeys. In primates, lateral geniculate nucleus (LGN) neurons form parallel pathways to the cortex, which are characterized by the expression of different calcium-binding proteins. LGN projections to the middle temporal (MT) area only originate in the koniocellular layers, where many neurons express calbindin. In contrast, projections to V1 also originate in the magnocellular and parvocellular layers, where neurons express parvalbumin but not calbindin. Our results demonstrate that this specificity is disrupted following long-term (1 to 3 y) unilateral V1 lesions, indicating active rearrangement of the geniculocortical circuit. In lesioned animals, retrograde tracing revealed MT-projecting neurons scattered throughout the lesion projection zone (LPZ, the sector of the LGN that underwent retrograde degeneration following a V1 lesion). Many of the MT-projecting neurons had large cell bodies and were located outside the koniocellular layers. Furthermore, we found that a large percentage of magno- and parvocellular neurons expressed calbindin in addition to the expected parvalbumin expression and that this coexpression was present in many of the MT-projecting neurons within the LPZ. These results demonstrate that V1 lesions trigger neurochemical and structural remodeling of the geniculo-extrastriate pathway, leading to the emergence of nonkoniocellular input to MT. This has potential implications for our understanding of the neurobiological bases of the residual visual abilities that survive V1 lesions, including motion perception and blindsight, and reveals targets for rehabilitation strategies to ameliorate the consequences of cortical blindness.},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
Here, we report on a previously unknown form of thalamocortical plasticity observed following lesions of the primary visual area (V1) in marmoset monkeys. In primates, lateral geniculate nucleus (LGN) neurons form parallel pathways to the cortex, which are characterized by the expression of different calcium-binding proteins. LGN projections to the middle temporal (MT) area only originate in the koniocellular layers, where many neurons express calbindin. In contrast, projections to V1 also originate in the magnocellular and parvocellular layers, where neurons express parvalbumin but not calbindin. Our results demonstrate that this specificity is disrupted following long-term (1 to 3 y) unilateral V1 lesions, indicating active rearrangement of the geniculocortical circuit. In lesioned animals, retrograde tracing revealed MT-projecting neurons scattered throughout the lesion projection zone (LPZ, the sector of the LGN that underwent retrograde degeneration following a V1 lesion). Many of the MT-projecting neurons had large cell bodies and were located outside the koniocellular layers. Furthermore, we found that a large percentage of magno- and parvocellular neurons expressed calbindin in addition to the expected parvalbumin expression and that this coexpression was present in many of the MT-projecting neurons within the LPZ. These results demonstrate that V1 lesions trigger neurochemical and structural remodeling of the geniculo-extrastriate pathway, leading to the emergence of nonkoniocellular input to MT. This has potential implications for our understanding of the neurobiological bases of the residual visual abilities that survive V1 lesions, including motion perception and blindsight, and reveals targets for rehabilitation strategies to ameliorate the consequences of cortical blindness.
25.
Chung et al.,
Comparative brain structure and visual processing in octopus from different habitats Journal Article
In: Current Biology , vol. 32, pp. 97–110, 2022, (University of Queensland).
Abstract | Links | BibTeX | Tags: Octopus
@article{nokey,
title = {Comparative brain structure and visual processing in octopus from different habitats},
author = {Chung et al., },
doi = {10.1016/j.cub.2021.10.070 },
year = {2022},
date = {2022-01-10},
urldate = {2022-01-10},
journal = {Current Biology },
volume = {32},
pages = {97–110},
abstract = {Different ecologies and behavioural differences influence the octopus central nervous system (CNS) which remains largely unknown. The publication presents phylogenetically informed comparison between diurnal and nocturnal coastal and a deep-sea species using brain imaging techniques. },
note = {University of Queensland},
keywords = {Octopus},
pubstate = {published},
tppubtype = {article}
}
Different ecologies and behavioural differences influence the octopus central nervous system (CNS) which remains largely unknown. The publication presents phylogenetically informed comparison between diurnal and nocturnal coastal and a deep-sea species using brain imaging techniques.
26.
Hinds LA, Henry S, Van De Weyer N, Robinson F, Ruscoe WA, Brown R.
Acute oral toxicity of zinc phosphide: an assessment for wildhouse mice (Musmusculus) Journal Article
In: 2022.
Abstract | Links | BibTeX | Tags: CSIRO, Mice
@article{nokey,
title = {Acute oral toxicity of zinc phosphide: an assessment for wildhouse mice (Musmusculus)},
author = {Hinds LA, Henry S, Van De Weyer N, Robinson F, Ruscoe WA, Brown R.},
url = {https://onlinelibrary.wiley.com/doi/epdf/10.1111/1749-4877.12666},
doi = {10.1111/1749-4877.12666},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-01},
abstract = {Irregular plagues of house mice, Mus musculus, incur major economic impacts on agricultural production in Australia. The efficacy of zinc phosphide (ZnP), the only registered broadacre control agent for mice, is reported as increasingly variable. Have mice become less sensitive over time or are they taking a sub-lethal dose and developing aversion? In this laboratory study, the sensitivity of mice (wild caught; outbred laboratory strain) was assessed using oral gavage of a range of ZnP concentrations. The estimated LD50values (72–79 mg ZnP/kg body weight)were similar for each mouse group but are significantly higher than previously reported. The willingness of mice to consume ZnP-coated grains was determined. ZnP-coated grains (50 g ZnP/kg grain) presented in the absence of alternative food were consumed and 94% of wild mice died. Mice provided with alternative food and ZnP-coated wheat grains (either 25 or 50 g ZnP/kg grain) consumed toxic and non-toxic grains, and mortality was lower(33–55%). If a sublethal amount of ZnP-coated grain was consumed, aversion occurred, mostly when alternative food was present. The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed. Under laboratory conditions, ZnP-coated grains coated with a new higher dose (50 g ZnP/kg grain) were readily consumed. Consumption of toxic grain occurred when alternative food was available but was decreased. Our unambiguous findings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.},
keywords = {CSIRO, Mice},
pubstate = {published},
tppubtype = {article}
}
Irregular plagues of house mice, Mus musculus, incur major economic impacts on agricultural production in Australia. The efficacy of zinc phosphide (ZnP), the only registered broadacre control agent for mice, is reported as increasingly variable. Have mice become less sensitive over time or are they taking a sub-lethal dose and developing aversion? In this laboratory study, the sensitivity of mice (wild caught; outbred laboratory strain) was assessed using oral gavage of a range of ZnP concentrations. The estimated LD50values (72–79 mg ZnP/kg body weight)were similar for each mouse group but are significantly higher than previously reported. The willingness of mice to consume ZnP-coated grains was determined. ZnP-coated grains (50 g ZnP/kg grain) presented in the absence of alternative food were consumed and 94% of wild mice died. Mice provided with alternative food and ZnP-coated wheat grains (either 25 or 50 g ZnP/kg grain) consumed toxic and non-toxic grains, and mortality was lower(33–55%). If a sublethal amount of ZnP-coated grain was consumed, aversion occurred, mostly when alternative food was present. The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed. Under laboratory conditions, ZnP-coated grains coated with a new higher dose (50 g ZnP/kg grain) were readily consumed. Consumption of toxic grain occurred when alternative food was available but was decreased. Our unambiguous findings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.
27.
Laura K Milton, Paul N Mirabella, Erika Greaves, David C Spanswick, Maarten van den Buuse, Brian J Oldfield , Claire J Foldi
Suppression of Corticostriatal Circuit Activity Improves Cognitive Flexibility and Prevents Body Weight Loss in Activity-Based Anorexia in Rats Journal Article
In: 2021, (Monash Biomedicine Discovery Institute, Monash University et al. ).
@article{nokey,
title = {Suppression of Corticostriatal Circuit Activity Improves Cognitive Flexibility and Prevents Body Weight Loss in Activity-Based Anorexia in Rats},
author = {Laura K Milton, Paul N Mirabella, Erika Greaves, David C Spanswick, Maarten van den Buuse, Brian J Oldfield , Claire J Foldi },
url = {https://pubmed.ncbi.nlm.nih.gov/32892984/},
year = {2021},
date = {2021-12-15},
urldate = {2021-12-15},
note = {Monash Biomedicine Discovery Institute, Monash University et al.
},
keywords = {Rats},
pubstate = {published},
tppubtype = {article}
}
28.
Mills, S.A, Jobling A.I., Dixon M.A., Fletcher E.L.
Fractalkine-induced microglial vasoregulation occurs within the retina and is altered early in diabetic retinopathy Journal Article
In: 2021, (University of Melbourne).
@article{nokey,
title = {Fractalkine-induced microglial vasoregulation occurs within the retina and is altered early in diabetic retinopathy},
author = {Mills, S.A, Jobling A.I., Dixon M.A., Fletcher E.L.},
url = {https://www.pnas.org/doi/10.1073/pnas.2112561118},
year = {2021},
date = {2021-12-13},
urldate = {2021-12-13},
note = {University of Melbourne},
keywords = {Mice},
pubstate = {published},
tppubtype = {article}
}
29.
Jan Henkel, Flavia Medeiros Savi, Arne Berner, Stephanie Fountain, Siamak Saifzadeh, Roland Steck, Devakar R Epari, Maria A Woodruff, Mark Knackstedt, Michael A Schuetz, Dietmar W Hutmacher
Scaffold-guided bone regeneration in large volume tibial segmental defects Journal Article
In: 2021, (Queensland University of Technology).
@article{nokey,
title = {Scaffold-guided bone regeneration in large volume tibial segmental defects},
author = {Jan Henkel, Flavia Medeiros Savi, Arne Berner, Stephanie Fountain, Siamak Saifzadeh, Roland Steck, Devakar R Epari, Maria A Woodruff, Mark Knackstedt, Michael A Schuetz, Dietmar W Hutmacher},
url = {https://pubmed.ncbi.nlm.nih.gov/34461285/},
doi = {10.1016/j.bone.2021.116163},
year = {2021},
date = {2021-12-01},
note = {Queensland University of Technology},
keywords = {Sheep},
pubstate = {published},
tppubtype = {article}
}
30.
Bakola S, Burman KJ, Bednarek S, Chan JM, Jermakow N, Worthy KK, Majka P, Rosa MGP.
In: 2021, (Monash University).
Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Afferent Connections of Cytoarchitectural Area 6M and Surrounding Cortex in the Marmoset: Putative Homologues of the Supplementary and Pre-supplementary Motor Areas},
author = {Bakola S, Burman KJ, Bednarek S, Chan JM, Jermakow N, Worthy KK, Majka P, Rosa MGP.},
url = {https://pubmed.ncbi.nlm.nih.gov/34255833/},
year = {2021},
date = {2021-11-23},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
31.
Wen Shi Lee, Arnold Reynaldi, Thakshila Amarasena, Miles P. Davenport, Matthew S. Parsons, Stephen J. Kent
Anti-Drug antibodies in pigtailed macaques receiving HIV broadly neutralising antibody PGT121 Journal Article
In: Frontiers in Immunology, vol. 12, 2021, (University of Melbourne).
Links | BibTeX | Tags: Macaque
@article{nokey,
title = {Anti-Drug antibodies in pigtailed macaques receiving HIV broadly neutralising antibody PGT121},
author = {Wen Shi Lee, Arnold Reynaldi, Thakshila Amarasena, Miles P. Davenport, Matthew S. Parsons and Stephen J. Kent},
doi = {10.3389/fimmu.2021.749891},
year = {2021},
date = {2021-11-21},
urldate = {2021-11-21},
journal = {Frontiers in Immunology},
volume = {12},
note = {University of Melbourne},
keywords = {Macaque},
pubstate = {published},
tppubtype = {article}
}
32.
Kirat K. Chand, Jatin Patel, S. T. Bjorkman, Seen-Ling Sim, Stephanie M. Miller, Elliot Teo, Lara Jones, Jane Sun, Paul B. Colditz, Kiarash Khosrotehrani, Julie A. Wixey
In: 2021, (University of Queensland).
Abstract | Links | BibTeX | Tags: piglets
@article{nokey,
title = {Combination of human endothelial colony-forming cells and mesenchymal stromal cells exert neuroprotective effects in the growth-restricted newborn},
author = {Kirat K. Chand, Jatin Patel, S. T. Bjorkman, Seen-Ling Sim, Stephanie M. Miller, Elliot Teo, Lara Jones, Jane Sun, Paul B. Colditz, Kiarash Khosrotehrani, Julie A. Wixey},
url = {https://www.nature.com/articles/s41536-021-00185-5.pdf},
doi = {https://doi.org/10.1038/s41536-021-00185-5},
year = {2021},
date = {2021-11-18},
urldate = {2021-11-18},
abstract = {The foetal brain is particularly vulnerable to the detrimental effects of foetal growth restriction (FGR) with subsequent abnormal neurodevelopment being common. There are no current treatments to protect the FGR newborn from lifelong neurological disorders. This study examines whether pure foetal mesenchymal stromal cells (MSC) and endothelial colony-forming cells (ECFC) from the human term placenta are neuroprotective through modulating neuroinflammation and supporting the brain vasculature. We determined that one dose of combined MSC-ECFCs (cECFC; 106 ECFC 106 MSC) on the first day of life to the newborn FGR piglet improved damaged vasculature, restored the neurovascular unit, reduced brain inflammation and improved adverse neuronal and white matter changes present in the FGR newborn piglet brain. These findings could not be reproduced using MSCs alone. These results demonstrate cECFC treatment exerts beneficial effects on multiple cellular components in the FGR brain and may act as a neuroprotectant.},
note = {University of Queensland},
keywords = {piglets},
pubstate = {published},
tppubtype = {article}
}
The foetal brain is particularly vulnerable to the detrimental effects of foetal growth restriction (FGR) with subsequent abnormal neurodevelopment being common. There are no current treatments to protect the FGR newborn from lifelong neurological disorders. This study examines whether pure foetal mesenchymal stromal cells (MSC) and endothelial colony-forming cells (ECFC) from the human term placenta are neuroprotective through modulating neuroinflammation and supporting the brain vasculature. We determined that one dose of combined MSC-ECFCs (cECFC; 106 ECFC 106 MSC) on the first day of life to the newborn FGR piglet improved damaged vasculature, restored the neurovascular unit, reduced brain inflammation and improved adverse neuronal and white matter changes present in the FGR newborn piglet brain. These findings could not be reproduced using MSCs alone. These results demonstrate cECFC treatment exerts beneficial effects on multiple cellular components in the FGR brain and may act as a neuroprotectant.
33.
Kwan, Chang, Yu, Mundinano, Fox, Homman-Ludiye, & Bourne
Visual cortical area MT is required for development of the dorsal stream and associated visuomotor behaviors Journal Article
In: 2021, (Monash University).
Abstract | Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Visual cortical area MT is required for development of the dorsal stream and associated visuomotor behaviors},
author = {Kwan, Chang, Yu, Mundinano, Fox, Homman-Ludiye, & Bourne},
url = {https://pubmed.ncbi.nlm.nih.gov/34417331/},
year = {2021},
date = {2021-09-29},
urldate = {2021-09-29},
abstract = {https://pubmed.ncbi.nlm.nih.gov/34417331/
},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
https://pubmed.ncbi.nlm.nih.gov/34417331/
34.
Fujihara, K.M. et al
Eprenetapopt triggers ferroptosis, inhibits NFS1 cysteine desulfurase, and synergizes with serine and glycine dietary restriction Journal Article
In: Science, vol. 8, iss. 37, 2021, (Peter MacCallum Cancer Centre, Melbourne).
@article{nokey,
title = {Eprenetapopt triggers ferroptosis, inhibits NFS1 cysteine desulfurase, and synergizes with serine and glycine dietary restriction},
author = {Fujihara, K.M. et al},
url = {https://www.science.org/doi/10.1126/sciadv.abm9427},
year = {2021},
date = {2021-09-14},
urldate = {2021-09-14},
journal = {Science},
volume = {8},
issue = {37},
note = {Peter MacCallum Cancer Centre, Melbourne},
keywords = {Mice},
pubstate = {published},
tppubtype = {article}
}
35.
Emran, AA. et al
A combination of epigenetic BET and CDK9 inhibitors for treatment of human melanoma Journal Article
In: 2021, (Centenary Institute).
@article{nokey,
title = { A combination of epigenetic BET and CDK9 inhibitors for treatment of human melanoma},
author = {Emran, AA. et al},
url = {https://www.jidonline.org/article/S0022-202X(21)01121-0/fulltext},
year = {2021},
date = {2021-09-01},
note = {Centenary Institute},
keywords = {Mice},
pubstate = {published},
tppubtype = {article}
}
36.
Jade L Thornton, Nicholas A Everett, Paige Webb, Anita J Turner, Jennifer L Cornish, Sarah J Baracz
Adolescent oxytocin administration reduces depression-like behaviour induced by early life stress in adult male and female rats Journal Article
In: 2021, (Macquarie University).
Abstract | Links | BibTeX | Tags: Rats
@article{nokey,
title = {Adolescent oxytocin administration reduces depression-like behaviour induced by early life stress in adult male and female rats},
author = {Jade L Thornton, Nicholas A Everett, Paige Webb, Anita J Turner, Jennifer L Cornish, Sarah J Baracz},
url = {https://pubmed.ncbi.nlm.nih.gov/33567331/},
doi = {10.1016/j.pnpbp.2021.110279},
year = {2021},
date = {2021-08-30},
urldate = {2021-08-30},
abstract = {Early life stress (ELS) exposure alters brain development, increasing vulnerability for mental illness in adulthood, including depression. Despite this association, there are no approved pharmacotherapies to protect against the emergence of mental illness resulting from ELS. Recent preclinical work showed that oxytocin (OT) administration in adulthood reduced depressive-like behaviour in male rats with a history of ELS. However, the ability of an OT treatment regime in adolescence, a critical developmental window for the OT system, to prevent the expression of depressive-like behaviours following ELS has not been investigated. Therefore, the present study aimed to determine whether chronic OT administration can ameliorate the enduring effects of ELS on depressive-like behaviours in both male and female rats. Following birth, Long Evans rat pups (N = 107) underwent maternal separation (MS) for either 15 min (MS15) or 6 h (MS360) on postnatal days (PND) 1-21. During adolescence (PND 28-42), rats received a daily injection of either OT (1 mg/kg) or saline. During adulthood (PND 57 onwards), effort-related motivation was measured using a model of effortful choice (EC), while behavioural despair was measured using the forced swim test (FST). Lastly, body and organ weights were measured to examine the physiological impacts of ELS and chronic OT administration. Overall, in both sexes, MS360 increased behavioural despair yet had no impact on effort-related motivation. Importantly, adolescent OT administration prevented the MS360-induced increase in behavioural despair in both males and females. Additionally, MS360 resulted in persistent reductions in body weight in both sexes post-weaning and increased spleen weight in males and adrenal weight in females. OT treatment had no impact on body weight in either sex, but prevented the MS-induced increase in adrenal gland weight in females. Overall, these findings have important implications for using oxytocin as a preventative pharmacotherapy after ELS.},
note = {Macquarie University},
keywords = {Rats},
pubstate = {published},
tppubtype = {article}
}
Early life stress (ELS) exposure alters brain development, increasing vulnerability for mental illness in adulthood, including depression. Despite this association, there are no approved pharmacotherapies to protect against the emergence of mental illness resulting from ELS. Recent preclinical work showed that oxytocin (OT) administration in adulthood reduced depressive-like behaviour in male rats with a history of ELS. However, the ability of an OT treatment regime in adolescence, a critical developmental window for the OT system, to prevent the expression of depressive-like behaviours following ELS has not been investigated. Therefore, the present study aimed to determine whether chronic OT administration can ameliorate the enduring effects of ELS on depressive-like behaviours in both male and female rats. Following birth, Long Evans rat pups (N = 107) underwent maternal separation (MS) for either 15 min (MS15) or 6 h (MS360) on postnatal days (PND) 1-21. During adolescence (PND 28-42), rats received a daily injection of either OT (1 mg/kg) or saline. During adulthood (PND 57 onwards), effort-related motivation was measured using a model of effortful choice (EC), while behavioural despair was measured using the forced swim test (FST). Lastly, body and organ weights were measured to examine the physiological impacts of ELS and chronic OT administration. Overall, in both sexes, MS360 increased behavioural despair yet had no impact on effort-related motivation. Importantly, adolescent OT administration prevented the MS360-induced increase in behavioural despair in both males and females. Additionally, MS360 resulted in persistent reductions in body weight in both sexes post-weaning and increased spleen weight in males and adrenal weight in females. OT treatment had no impact on body weight in either sex, but prevented the MS-induced increase in adrenal gland weight in females. Overall, these findings have important implications for using oxytocin as a preventative pharmacotherapy after ELS.
37.
Isaac M. Barber-Axthelm, Hannah G. Kelly, Robyn Esterbauer, Kathleen M. Wragg, Anne M. Gibbon, Wen Shi Lee, Adam K. Wheatley, Stephen J. Kent, Hyon-Xhi Tan, and Jennifer A. Juno C
Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node Journal Article
In: The Journal of Immunology, vol. 207, iss. 2, pp. 735-744, 2021, (Macaques – Monash Animal Research Platform Gippsland Field Station; Mice University of Melbourne).
Abstract | Links | BibTeX | Tags: Macaque, Mice
@article{nokey,
title = {Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node},
author = {Isaac M. Barber-Axthelm, Hannah G. Kelly, Robyn Esterbauer, Kathleen M. Wragg,
Anne M. Gibbon, Wen Shi Lee, Adam K. Wheatley, Stephen J. Kent, Hyon-Xhi Tan,
and Jennifer A. Juno
C},
doi = { https://doi.org/10.4049/jimmunol.2001299},
year = {2021},
date = {2021-07-15},
urldate = {2021-07-15},
journal = {The Journal of Immunology},
volume = {207},
issue = {2},
pages = {735-744},
abstract = {Characterization of germinal center B and T cell responses yields critical insights into vaccine immunogenicity. Nonhuman primates are a key preclinical animal model for human vaccine development, allowing both lymph node (LN) and circulating immune responses to be longitudinally sampled for correlates of vaccine efficacy. However, patterns of vaccine Ag drainage via the lymphatics after i.m. immunization can be stochastic, driving uneven deposition between lymphoid sites and between individual LN within larger clusters. To improve the accurate isolation of Ag-exposed LN during biopsies and necropsies, we developed and validated a method for coformulating candidate vaccines with tattoo ink in both mice and pigtail macaques. This method allowed for direct visual identification of vaccine-draining LN and evaluation of relevant Ag-specific B and T cell responses by flow cytometry. This approach is a significant advancement in improving the assessment of vaccine-induced immunity in highly relevant nonhuman primate models.},
note = {Macaques - Monash Animal Research Platform Gippsland Field Station; Mice University of Melbourne},
keywords = {Macaque, Mice},
pubstate = {published},
tppubtype = {article}
}
Characterization of germinal center B and T cell responses yields critical insights into vaccine immunogenicity. Nonhuman primates are a key preclinical animal model for human vaccine development, allowing both lymph node (LN) and circulating immune responses to be longitudinally sampled for correlates of vaccine efficacy. However, patterns of vaccine Ag drainage via the lymphatics after i.m. immunization can be stochastic, driving uneven deposition between lymphoid sites and between individual LN within larger clusters. To improve the accurate isolation of Ag-exposed LN during biopsies and necropsies, we developed and validated a method for coformulating candidate vaccines with tattoo ink in both mice and pigtail macaques. This method allowed for direct visual identification of vaccine-draining LN and evaluation of relevant Ag-specific B and T cell responses by flow cytometry. This approach is a significant advancement in improving the assessment of vaccine-induced immunity in highly relevant nonhuman primate models.
38.
Aridas, et al
Melatonin augments the neuroprotective effects of hypothermia in lambs following perinatal asphyxia Journal Article
In: 2021, (Monash University, Hudson Institute of Medical Research).
@article{nokey,
title = {Melatonin augments the neuroprotective effects of hypothermia in lambs following perinatal asphyxia},
author = {Aridas, et al },
url = {https://pubmed.ncbi.nlm.nih.gov/34032315/},
year = {2021},
date = {2021-06-20},
note = {Monash University, Hudson Institute of Medical Research},
keywords = {Sheep},
pubstate = {published},
tppubtype = {article}
}
39.
Liu Y, Long x, Martin PR, Solomon SG, Gong P.
Lévy walk dynamics explain gamma burst patterns in primate cerebral cortex Journal Article
In: 2021, (University of Sydney).
Links | BibTeX | Tags: Non-human primates
@article{nokey,
title = {Lévy walk dynamics explain gamma burst patterns in primate cerebral cortex },
author = {Liu Y, Long x, Martin PR, Solomon SG, Gong P.},
url = {https://pubmed.ncbi.nlm.nih.gov/34131276/},
year = {2021},
date = {2021-06-15},
note = {University of Sydney},
keywords = {Non-human primates},
pubstate = {published},
tppubtype = {article}
}
40.
Jordan C. Conway, Rema A. Oliver, Tian Wang, Daniel J. Wills, Joe Herbert, Tom Buckland, William R. Walsh, Iain R. Gibson
The efficacy of a nanosynthetic bone graft substitute as a bone graft extender in rabbit posterolateral fusion Journal Article
In: vol. 21, pp. 1925-1937, 2021, (University of New South Wales).
@article{,
title = {The efficacy of a nanosynthetic bone graft substitute as a bone graft extender in rabbit posterolateral fusion},
author = {Jordan C. Conway, Rema A. Oliver, Tian Wang, Daniel J. Wills, Joe Herbert, Tom Buckland, William R. Walsh, Iain R. Gibson},
doi = {10.1016/j.spinee.2021.05.017},
year = {2021},
date = {2021-05-19},
urldate = {2021-05-19},
volume = {21},
pages = {1925-1937},
publisher = {The Spine Journal},
note = {University of New South Wales},
keywords = {},
pubstate = {published},
tppubtype = {article}
}