Publications: Australian Animal Experimentation

@article{nokey,

title = {The role of frontopolar cortex in adjusting the balance between response  execution and action inhibition in anthropoids},

author = {Azadeh Feizpour, Mark J. Buckley, Inaki C. Mundinano, Marcello G.P. Rosa, Farshad Alizadeh Mansouri},

doi = {https://doi.org/10.1016/j.pneurobio.2024.102671},

year  = {2024},

date = {2024-10-05},

urldate = {2024-10-05},

journal = {Progress in Neurobiology},

volume = {241},

number = {102671},

abstract = {Executive control of behaviour entails keeping a fine balance between response execution and action inhibition. The most anterior part of the prefrontal cortex (frontopolar cortex) is highly developed in anthropoids; however, no previous study has examined its essential (indispensable) role in regulating the interplay between action execution and inhibition. In this cross-species study, we examine the performance of humans and macaque monkeys in the context of a stop-signal task and then assess the consequence of selective and bilateral damage to frontopolar cortex on monkeys’ behaviour. Humans and monkeys showed significant within-session practice related adjustments in both response execution (increase in response time (RT) and decrease in response variabilities) and action inhibition (enhanced inhibition). Furthermore, both species expressed context-dependent (post-error and post-stop) behavioral adjustments. In post-lesion testing, frontopolar-damaged monkeys had a 

longer RT and lower percentage of timeout trials, compared to their pre-lesion performance. The practice-related changes in mean RT and in RT variability were significantly heightened in frontopolar-damaged monkeys. They also showed attenuated post-error, but exaggerated post-stop, behavioural adjustments. Importantly, frontopolar damage had no significant effects on monkeys’ inhibition ability. Our findings indicate that frontopolar cortex plays a critical role in allocation of control to response execution, but not action inhibition.},

note = {Monash University},

keywords = {Macaque, Non-human primates},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Oral nanotherapeutic formulation of insulin with reduced episodes of hypoglycaemia},

author = {Nicholas J. Hunt, Glen P. Lockwood, Scott J. Heffernan, Jarryd Daymond, Meng Ngu, Ramesh K. Narayanan, Lara J. Westwood, Biswaranjan Mohanty, Lars Esser, Charlotte C. Williams, Zdenka Kuncic, Peter A. G. McCourt, David G. Le Couteur, Victoria C. Cogger},

url = {https://www.nature.com/articles/s41565-023-01565-2},

doi = {10.1038/s41565-023-01565-2},

year  = {2024},

date = {2024-01-02},

urldate = {2024-01-02},

journal = {Nat. Nanotechnol.},

volume = {19},

pages = {534–544},

abstract = {Injectable insulin is an extensively used medication with potential life-threatening hypoglycaemic events. Here we report on insulin-conjugated silver sulfide quantum dots coated with a chitosan/glucose polymer to produce a responsive oral insulin nanoformulation. This formulation is pH responsive, is insoluble in acidic environments and shows increased absorption in human duodenum explants and Caenorhabditis elegans at neutral pH. The formulation is sensitive to glucosidase enzymes to trigger insulin release. It is found that the formulation distributes to the liver in mice and rats after oral administration and promotes a dose-dependent reduction in blood glucose without promoting hypoglycaemia or weight gain in diabetic rodents. Non-diabetic baboons also show a dose-dependent reduction in blood glucose. No biochemical or haematological toxicity or adverse events were observed in mice, rats and non-human primates. The formulation demonstrates the potential to orally control blood glucose without hypoglycaemic episodes.},

note = {The University of Sydney, Camperdown, New South Wales, Australia},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Superior efficacy of a skin-applied microprojection device for delivering a novel Zika DNA vaccine},

author = {Wijesundara, Danushka K. et al.},

url = {https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(23)00274-3},

doi = {https://doi.org/10.1016/j.omtn.2023.102056},

year  = {2023},

date = {2023-12-12},

urldate = {2023-12-12},

journal = {Molecular Therapy },

volume = {34},

abstract = {Zika virus (ZIKV) infections are spreading silently with limited global surveillance in at least 89 countries and territories. There is a pressing need to develop an effective vaccine suitable for equitable distribution globally. Consequently, we previously developed a proprietary DNA vaccine encoding secreted non-structural protein 1 of ZIKV (pVAX-tpaNS1) to elicit rapid protection in a T cell-dependent manner in mice. In the current study, we evaluated the stability, efficacy, and immunogenicity of delivering this DNA vaccine into the skin using a clinically effective and proprietary high-density microarray patch (HD-MAP). Dry-coating of pVAX-tpaNS1 on the HD-MAP device resulted in no loss of vaccine stability at 40°C storage over the course of 28 days. Vaccination of mice (BALB/c) with the HD-MAP-coated pVAX-tpaNS1 elicited a robust anti-NS1 IgG response in both the cervicovaginal mucosa and systemically and afforded protection against live ZIKV challenge. Furthermore, the vaccination elicited a significantly higher magnitude and broader NS1-specific T helper and cytotoxic T cell response in vivo compared with traditional needle and syringe intradermal vaccination. Overall, the study highlights distinctive immunological advantages coupled with an excellent thermostability profile of using the HD-MAP device to deliver a novel ZIKV DNA vaccine},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {H3K4me3 remodeling induced acquired resistance through O-GlcNAc transferase},

author = {Menon, D.R. et al},

url = {https://www.sciencedirect.com/science/article/pii/S1368764623000766?via%3Dihub

},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {Drug Resistance Updates},

volume = {71},

note = {University of Queensland},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Lymph node targeted multi-epitope subunit vaccine promotes effective immunity to EBV in HLA-expressing mice},

author = {Vijayendra Dasari, et al },

doi = {https://doi.org/10.1038/s41467-023-39770-1},

year  = {2023},

date = {2023-08-08},

journal = {Nature Communications},

volume = {14},

number = {4371},

note = {QIMR Berghofer Medical Research Institute, Brisbane, Australia},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Modifying effects of sodium selenate in a model of drug resistant, temporal lobe epilepsy},

author = {Pablo Miguel Casillas-Espinosa, Alison Anderson, Anna Harutyunyan, Crystal Li, Jiyoon Lee, Emma L Braine, Rhys D Brady, Mujun Sun, Cheng Huang, Christopher K Barlow, Anup D Shah, Ralf B Schittenhelm, Richelle Mychasiuk, Nigel C Jones, Sandy R Shultz, Terence J O’Brien },

url = {https://elifesciences.org/articles/78877},

doi = { https://doi.org/10.7554/eLife.78877},

issn = {2050-084X},

year  = {2023},

date = {2023-03-09},

urldate = {2023-03-09},

journal = {eLife Sciences},

keywords = {Rats},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A repeated measures cognitive affective bias test in rats: comparison with forced swim test},

author = {Benjamin Aliphon ,Twain Dai, Jessica Moretti, Marissa Penrose-Menz, Wilhelmina H A M Mulders, Dominique Blache, Jennifer Rodger},

url = {https://pubmed.ncbi.nlm.nih.gov/36450831/},

doi = {10.1007/s00213-022-06281-8},

year  = {2022},

date = {2022-12-01},

urldate = {2022-12-01},

journal = {Psychopharmacology (Berl) },

abstract = {Objectives: This study aimed to validate a repeated measures cognitive affective bias (CAB) test in a rat model of chronic stress and compare CAB with forced swim test (FST) measures.

Method: Male and female Sprague Dawley rats were trained to associate large and small rewards with scent, spatial, and tactile cues, and their response to an ambiguous tactile stimulus tested. Rats underwent weekly CAB testing for 4 weeks with no intervention, or for 2 weeks of chronic restraint stress (CRS), followed by 2 weeks of fluoxetine, vehicle, or no treatment. CRS rats also underwent the FST at selected timepoints.},

note = {University of Western Australia},

keywords = {Rats},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effects of an enhanced acoustic environment on residual hearing following chronic cochlear implantation and electrical stimulation in the partially deafened cat},

author = {Wise, AK., Atkinson, P, Fallon, J.B.},

url = {https://www.sciencedirect.com/science/article/pii/S0378595522002039?via%3Dihub},

doi = {https://doi.org/10.1016/j.heares.2022.108635},

year  = {2022},

date = {2022-12-01},

urldate = {2022-12-01},

journal = {Hearing Research},

volume = {426},

note = {The Bionics Institute, University of Melbourne Royal Victorian Eye and Ear Hospital},

keywords = {cats, kittens},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Optogenetic restoration of high sensitivity vision with bReaChES, a red-shifted channelrhodopsin},

author = {Lay Khoon Too, Weiyong Shen, Dario A. Protti, Atomu Sawatari, Dylan A. Black, Catherine A. Leamey, Jin Y. Huang, So-Ra Lee, Ashish E. Mathai, Leszek Lisowski, John Y. Lin, Mark C. Gillies & Matthew P. Simunovic },

doi = {https://doi.org/10.1038/s41598-022-23572-4},

year  = {2022},

date = {2022-11-11},

urldate = {2022-11-11},

journal = {Nature - Scientific Reports},

volume = {12},

number = {19312},

note = {University of Sydney},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Orientation pinwheels in primary visual cortex of a highly visual marsupial},

author = {Young Jun Jung, Ali Almasi, Shi H. Sun, Molis Yunzab, Shaun L. Cloherty,

Sebastien H. Bauquier, Marilyn Renfree, Hamish Meffin, Michael R. Ibbotson},

editor = {Sci. Adv. },

doi = {doi/10.1126/sciadv.abn0954},

year  = {2022},

date = {2022-09-30},

urldate = {2022-09-30},

journal = {Science Advances},

volume = {8},

abstract = {Primary visual cortices in many mammalian species exhibit modular and periodic orientation preference maps arranged in pinwheel-like layouts. The role of inherited traits as opposed to environmental influences in determining this organization remains unclear. Here, we characterize the cortical organization of an Australian marsupial, revealing pinwheel organization resembling that of eutherian carnivores and primates but distinctly different from the simpler salt-and-pepper arrangement of eutherian rodents and rabbits. The divergence of marsupials from eutherians 160 million years ago and the later emergence of rodents and rabbits suggest that the salt-and pepper structure is not the primitive ancestral form. Rather, the genetic code that enables complex pinwheel formation is likely widespread, perhaps extending back to the common therian ancestors of modern mammals.},

note = {University of Melbourne, Wallabies},

keywords = {wallabies},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Impact of High Fat Consumption on Neurological Functions after Traumatic Brain Injury in Rats},

author = {Shannon Thomson, Yik Lung Chan, Chenju Yi Baoming Wang, Rita Machaalani, Brian G Oliver Catherine A Gorrie, Hui Chen },

url = {https://pubmed.ncbi.nlm.nih.gov/35658673/},

year  = {2022},

date = {2022-07-14},

urldate = {2022-07-14},

note = {University of Technology Sydney},

keywords = {Rats},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Sustained endosomal release of a neurokinin-1 receptor antagonist from nanostars provides long-lasting relief of chronic pain},

author = {Nicholas A. Veldhuis et al },

url = {https://research.monash.edu/en/publications/sustained-endosomal-release-of-a-neurokinin-1-receptor-antagonist},

doi = {10.1016/j.biomaterials.2022.121536},

year  = {2022},

date = {2022-06-30},

urldate = {2022-06-30},

journal = {Biomaterials },

volume = {285},

abstract = {Soft polymer nanoparticles designed to disassemble and release an antagonist of the neurokinin 1 receptor (NK1R) in endosomes provide efficacious yet transient relief from chronic pain. These micellar nanoparticles are unstable and rapidly release cargo, which may limit the duration of analgesia. We examined the efficacy of stable star polymer nanostars containing the NK1R antagonist aprepitant-amine for the treatment of chronic pain in mice. Nanostars continually released cargo for 24 h, trafficked through the endosomal system, and disrupted NK1R endosomal signaling. After intrathecal injection, nanostars accumulated in endosomes of spinal neurons. Nanostar-aprepitant reversed mechanical, thermal and cold allodynia and normalized nociceptive behavior more efficaciously than free aprepitant in preclinical models of neuropathic and inflammatory pain. Analgesia was maintained for >10 h. The sustained endosomal delivery of antagonists from slow-release nanostars provides effective and long-lasting reversal of chronic pain.},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Altered amyloid precursor protein, tau-regulatory proteins, neuronal numbers and behaviour, but no tau pathology, synaptic and inflammatory changes or memory deficits, at 1 month following repetitive mild traumatic brain injury},

author = {Sydney M.A. Juan, Maria Daglas, Paul A. Adlard},

doi = {10.1111/ejn.15752},

year  = {2022},

date = {2022-06-29},

urldate = {2022-06-29},

journal = {European Journal of Neuroscience},

abstract = {Repetitive mild traumatic brain injury, commonly experienced following sports injuries, results in various secondary injury processes and is increasingly recognised as a risk factor for the development of neurodegenerative conditions such as chronic traumatic encephalopathy, which is characterised by tau pathology. We aimed to characterise the underlying pathological mechanisms that might contribute to the onset of neurodegeneration and behavioural changes in the less-explored subacute (1-month) period following single or repetitive controlled cortical impact injury (five impacts, 48 h apart) in 12-week-old male and female C57Bl6 mice. We conducted motor and cognitive testing, extensively characterised the status of tau and its regulatory proteins via western blot and quantified neuronal populations using stereology. We report that r-mTBI resulted in neurobehavioural deficits, gait impairments and anxiety-like behaviour at 1 month post-injury, effects not seen following a single injury. R-mTBI caused a significant increase in amyloid precursor protein, an increased trend towards tau phosphorylation and significant changes in kinase/phosphatase proteins that may promote a downstream increase in tau phosphorylation, but no changes in synaptic or neuroinflammatory markers. Lastly, we report neuronal loss in various brain regions following both single and repeat injuries. We demonstrate herein that repeated impacts are required to promote the initiation of a cascade of biochemical events that are consistent with the onset of neurodegeneration subacutely post-injury. Identifying the timeframe in which these changes occur and the pathological mechanisms involved will be crucial for the development of future therapeutics to prevent the onset or mitigate the progression of neurodegeneration following r-mTBI.},

note = {The Florey Institute of Neuroscience & Mental Health, The Melbourne Dementia Research Centre, the University of Melbourne.},

keywords = {Mice},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = { Replicating infant-specific reactive astrocyte functions in the injured adult brain},

author = {Leon Teo, Anthony G Boghdadi, Jihane Homman-Ludiye, Inaki-Carril Mundinano, William C Kwan, James A Bourne},

url = {https://pubmed.ncbi.nlm.nih.gov/34147584/},

doi = {10.1016/j.pneurobio.2021.102108},

year  = {2022},

date = {2022-06-17},

urldate = {2022-06-17},

abstract = {Infants and adults respond differently to brain injuries. Specifically, improved neuronal sparing along with reduced astrogliosis and glial scarring often observed earlier in life, likely contributes to improved long-term outcomes. Understanding the underlying mechanisms could enable the recapitulation of neuroprotective effects, observed in infants, to benefit adults after brain injuries. We reveal that in primates, Eph/ ephrin signaling contributes to age-dependent reactive astrocyte behavior. Ephrin-A5 expression on astrocytes was more protracted in adults, whereas ephrin-A1 was only expressed on infant astrocytes. Furthermore, ephrin-A5 exacerbated major hallmarks of astrocyte reactivity via EphA2 and EphA4 receptors, which was subsequently alleviated by ephrin-A1. Rather than suppressing reactivity, ephrin-A1 signaling shifted astrocytes towards GAP43+ neuroprotection, accounting for improved neuronal sparing in infants. Reintroducing ephrin-A1 after middle-aged focal ischemic injury significantly attenuated glial scarring, improved neuronal sparing and preserved circuitry. Therefore, beneficial infant mechanisms can be recapitulated in adults to improve outcomes after CNS injuries.},

note = {Monash University},

keywords = {Marmosets, Non-human primates},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Xenotransplantation of Genetically Modified Neonatal Pig Islets Cures Diabetes in Baboons},

author = {Hawthorn, WJ et al},

doi = {https://doi.org/10.3389/fimmu.2022.898948},

year  = {2022},

date = {2022-06-16},

urldate = {2023-06-16},

journal = {Frontiers in Immunology},

volume = {13},

abstract = {Xenotransplantation using porcine donors is rapidly approaching clinical applicability as an alternative therapy for treatment of many end-stage diseases including type 1 diabetes. Porcine neonatal islet cell clusters (NICC) have normalised blood sugar levels for relatively short periods in the preclinical diabetic rhesus model but have met with limited success in the stringent baboon model. Here we report that NICC from genetically modified (GM) pigs deleted for αGal and expressing the human complement regulators CD55 and CD59 can cure diabetes long-term in immunosuppressed baboons, with maximum graft survival exceeding 22 months. Five diabetic baboons were transplanted intraportally with 9,673 – 56,913 islet equivalents (IEQ) per kg recipient weight. Immunosuppression consisted of T cell depletion with an anti-CD2 mAb, tacrolimus for the first 4 months, and maintenance with belatacept and anti-CD154; no anti-inflammatory treatment or cytomegalovirus (CMV) prophylaxis/treatment was given. This protocol was well tolerated, with all recipients maintaining or gaining weight. Recipients became insulin-independent at a mean of 87 ± 43 days post-transplant and remained insulin-independent for 397 ± 174 days. Maximum graft survival was 675 days. Liver biopsies showed functional islets staining for all islet endocrine components, with no evidence of the inflammatory blood-mediated inflammatory reaction (IBMIR) and minimal leukocytic infiltration. The costimulation blockade-based immunosuppressive protocol prevented an anti-pig antibody response in all recipients. In conclusion, we demonstrate that genetic modification of the donor pig enables attenuation of early islet xenograft injury, and in conjunction with judicious immunosuppression provides excellent long-term function and graft survival in the diabetic baboon model.},

note = {Westmead Institute for Medical Research, NSW.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Topical wound anaesthesia: efficacy to mitigate piglet castration pain},

author = {Sheil, ML, Chambers, M and Sharpe, B.},

url = {https://pubmed.ncbi.nlm.nih.gov/32096229/},

doi = {doi: 10.1111/avj.12930},

year  = {2022},

date = {2022-06-01},

urldate = {2022-06-01},

journal = {Aust Vet Journal},

volume = {98},

issue = {6},

pages = {256-263},

note = {Piglets},

keywords = {piglets},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A Preclinical Study of Standard Versus Accelerated Transcranial Magnetic Stimulation for Depression in Adolescents},

author = {Bhedita J. Seewoo, Lauren A. Hennessy, Liz A. Jaeschke, Leah A. Mackie, Sarah J. Etherington, Sarah A. Dunlop, Paul E. Croarkin, and Jennifer Rodger},

doi = {10.1089/cap.2021.0100},

year  = {2022},

date = {2022-04-18},

urldate = {2022-04-18},

volume = {32},

number = {3},

abstract = {Abstract

Objective: Ongoing studies are focused on adapting transcranial magnetic stimulation (TMS) for the treatment of major depressive disorder in adolescent humans. Most protocols in adolescent humans to date have delivered daily 10 Hz prefrontal stimulation with mixed results. Novel TMS dosing strategies such as accelerated TMS have recently been considered. There are knowledge gaps related to the potential clinical and pragmatic advantages of accelerated TMS. This pilot study compared the behavioral effects of a standard daily and accelerated low-intensity TMS (LI-TMS) protocol in an adolescent murine model of depression.



Methods: Male adolescent Sprague Dawley rats were placed in transparent plexiglass tubes for 2.5 hours daily for 13 days as part of a study to validate the chronic restraint stress (CRS) protocol. Rats subsequently received 10 minutes of active or sham 10 Hz LI-TMS daily for 2 weeks (standard) or three times daily for 1 week (accelerated). Behavior was assessed using the elevated plus maze and forced swim test (FST). Hippocampal neurogenesis was assessed by injection of the thymidine analogue 5-ethynyl-2′-deoxyuridine at the end of LI-TMS treatment (2 weeks standard, 1 week accelerated), followed by postmortem histological analysis.},

note = {University of Western Australia, Perron Institute for Neurological & Translational Science, WA},

keywords = {Rats},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Rapid GFAP and Iba1 expression changes in the female rat brain following spinal cord injury},

author = {Mawj Mandwie, Jordan A Piper, Catherine A Gorrie, Kevin A Keay, Giuseppe Musumeci , Ghaith Al-Badri, Alessandro Castorina},

url = {https://pubmed.ncbi.nlm.nih.gov/34269213/},

year  = {2022},

date = {2022-02-17},

urldate = {2022-02-17},

note = {University of Technology Sydney},

keywords = {Rats},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Remodeling of lateral geniculate nucleus projections to extrastriate area MT following long-term lesions of striate cortex},

author = {Nafiseh Atapour, Katrina H Worthy, Marcello G P Rosa},

url = {https://pubmed.ncbi.nlm.nih.gov/35058366/},

doi = {10.1073/pnas.2117137119},

year  = {2022},

date = {2022-01-25},

urldate = {2022-01-25},

abstract = {Here, we report on a previously unknown form of thalamocortical plasticity observed following lesions of the primary visual area (V1) in marmoset monkeys. In primates, lateral geniculate nucleus (LGN) neurons form parallel pathways to the cortex, which are characterized by the expression of different calcium-binding proteins. LGN projections to the middle temporal (MT) area only originate in the koniocellular layers, where many neurons express calbindin. In contrast, projections to V1 also originate in the magnocellular and parvocellular layers, where neurons express parvalbumin but not calbindin. Our results demonstrate that this specificity is disrupted following long-term (1 to 3 y) unilateral V1 lesions, indicating active rearrangement of the geniculocortical circuit. In lesioned animals, retrograde tracing revealed MT-projecting neurons scattered throughout the lesion projection zone (LPZ, the sector of the LGN that underwent retrograde degeneration following a V1 lesion). Many of the MT-projecting neurons had large cell bodies and were located outside the koniocellular layers. Furthermore, we found that a large percentage of magno- and parvocellular neurons expressed calbindin in addition to the expected parvalbumin expression and that this coexpression was present in many of the MT-projecting neurons within the LPZ. These results demonstrate that V1 lesions trigger neurochemical and structural remodeling of the geniculo-extrastriate pathway, leading to the emergence of nonkoniocellular input to MT. This has potential implications for our understanding of the neurobiological bases of the residual visual abilities that survive V1 lesions, including motion perception and blindsight, and reveals targets for rehabilitation strategies to ameliorate the consequences of cortical blindness.},

note = {Monash University},

keywords = {Marmosets, Non-human primates},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Comparative brain structure and visual processing in octopus from different habitats},

author = {Chung et al., },

doi = {10.1016/j.cub.2021.10.070 },

year  = {2022},

date = {2022-01-10},

urldate = {2022-01-10},

journal = {Current Biology },

volume = {32},

pages = {97–110},

abstract = {Different ecologies and behavioural differences influence the octopus central nervous system (CNS) which remains largely unknown. The publication presents phylogenetically informed comparison between diurnal and nocturnal coastal and a deep-sea species using brain imaging techniques. },

note = {University of Queensland},

keywords = {Octopus},

pubstate = {published},

tppubtype = {article}

}