This page includes a summary of recent publications (2019 onwards). This list is not exhaustive but a summary of the types of experimentation to which animals are subjected in Australian laboratories. More detailed profiles of Australian animal research can be found in our case studies.
1.
Leon Teo, Anthony G Boghdadi, Jihane Homman-Ludiye, Inaki-Carril Mundinano, William C Kwan, James A Bourne
Replicating infant-specific reactive astrocyte functions in the injured adult brain Journal Article
In: 2022, (Monash University).
Abstract | Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = { Replicating infant-specific reactive astrocyte functions in the injured adult brain},
author = {Leon Teo, Anthony G Boghdadi, Jihane Homman-Ludiye, Inaki-Carril Mundinano, William C Kwan, James A Bourne},
url = {https://pubmed.ncbi.nlm.nih.gov/34147584/},
doi = {10.1016/j.pneurobio.2021.102108},
year = {2022},
date = {2022-06-17},
urldate = {2022-06-17},
abstract = {Infants and adults respond differently to brain injuries. Specifically, improved neuronal sparing along with reduced astrogliosis and glial scarring often observed earlier in life, likely contributes to improved long-term outcomes. Understanding the underlying mechanisms could enable the recapitulation of neuroprotective effects, observed in infants, to benefit adults after brain injuries. We reveal that in primates, Eph/ ephrin signaling contributes to age-dependent reactive astrocyte behavior. Ephrin-A5 expression on astrocytes was more protracted in adults, whereas ephrin-A1 was only expressed on infant astrocytes. Furthermore, ephrin-A5 exacerbated major hallmarks of astrocyte reactivity via EphA2 and EphA4 receptors, which was subsequently alleviated by ephrin-A1. Rather than suppressing reactivity, ephrin-A1 signaling shifted astrocytes towards GAP43+ neuroprotection, accounting for improved neuronal sparing in infants. Reintroducing ephrin-A1 after middle-aged focal ischemic injury significantly attenuated glial scarring, improved neuronal sparing and preserved circuitry. Therefore, beneficial infant mechanisms can be recapitulated in adults to improve outcomes after CNS injuries.},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
Infants and adults respond differently to brain injuries. Specifically, improved neuronal sparing along with reduced astrogliosis and glial scarring often observed earlier in life, likely contributes to improved long-term outcomes. Understanding the underlying mechanisms could enable the recapitulation of neuroprotective effects, observed in infants, to benefit adults after brain injuries. We reveal that in primates, Eph/ ephrin signaling contributes to age-dependent reactive astrocyte behavior. Ephrin-A5 expression on astrocytes was more protracted in adults, whereas ephrin-A1 was only expressed on infant astrocytes. Furthermore, ephrin-A5 exacerbated major hallmarks of astrocyte reactivity via EphA2 and EphA4 receptors, which was subsequently alleviated by ephrin-A1. Rather than suppressing reactivity, ephrin-A1 signaling shifted astrocytes towards GAP43+ neuroprotection, accounting for improved neuronal sparing in infants. Reintroducing ephrin-A1 after middle-aged focal ischemic injury significantly attenuated glial scarring, improved neuronal sparing and preserved circuitry. Therefore, beneficial infant mechanisms can be recapitulated in adults to improve outcomes after CNS injuries.
2.
Nafiseh Atapour, Katrina H Worthy, Marcello G P Rosa
Remodeling of lateral geniculate nucleus projections to extrastriate area MT following long-term lesions of striate cortex Journal Article
In: 2022, (Monash University).
Abstract | Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Remodeling of lateral geniculate nucleus projections to extrastriate area MT following long-term lesions of striate cortex},
author = {Nafiseh Atapour, Katrina H Worthy, Marcello G P Rosa},
url = {https://pubmed.ncbi.nlm.nih.gov/35058366/},
doi = {10.1073/pnas.2117137119},
year = {2022},
date = {2022-01-25},
urldate = {2022-01-25},
abstract = {Here, we report on a previously unknown form of thalamocortical plasticity observed following lesions of the primary visual area (V1) in marmoset monkeys. In primates, lateral geniculate nucleus (LGN) neurons form parallel pathways to the cortex, which are characterized by the expression of different calcium-binding proteins. LGN projections to the middle temporal (MT) area only originate in the koniocellular layers, where many neurons express calbindin. In contrast, projections to V1 also originate in the magnocellular and parvocellular layers, where neurons express parvalbumin but not calbindin. Our results demonstrate that this specificity is disrupted following long-term (1 to 3 y) unilateral V1 lesions, indicating active rearrangement of the geniculocortical circuit. In lesioned animals, retrograde tracing revealed MT-projecting neurons scattered throughout the lesion projection zone (LPZ, the sector of the LGN that underwent retrograde degeneration following a V1 lesion). Many of the MT-projecting neurons had large cell bodies and were located outside the koniocellular layers. Furthermore, we found that a large percentage of magno- and parvocellular neurons expressed calbindin in addition to the expected parvalbumin expression and that this coexpression was present in many of the MT-projecting neurons within the LPZ. These results demonstrate that V1 lesions trigger neurochemical and structural remodeling of the geniculo-extrastriate pathway, leading to the emergence of nonkoniocellular input to MT. This has potential implications for our understanding of the neurobiological bases of the residual visual abilities that survive V1 lesions, including motion perception and blindsight, and reveals targets for rehabilitation strategies to ameliorate the consequences of cortical blindness.},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
Here, we report on a previously unknown form of thalamocortical plasticity observed following lesions of the primary visual area (V1) in marmoset monkeys. In primates, lateral geniculate nucleus (LGN) neurons form parallel pathways to the cortex, which are characterized by the expression of different calcium-binding proteins. LGN projections to the middle temporal (MT) area only originate in the koniocellular layers, where many neurons express calbindin. In contrast, projections to V1 also originate in the magnocellular and parvocellular layers, where neurons express parvalbumin but not calbindin. Our results demonstrate that this specificity is disrupted following long-term (1 to 3 y) unilateral V1 lesions, indicating active rearrangement of the geniculocortical circuit. In lesioned animals, retrograde tracing revealed MT-projecting neurons scattered throughout the lesion projection zone (LPZ, the sector of the LGN that underwent retrograde degeneration following a V1 lesion). Many of the MT-projecting neurons had large cell bodies and were located outside the koniocellular layers. Furthermore, we found that a large percentage of magno- and parvocellular neurons expressed calbindin in addition to the expected parvalbumin expression and that this coexpression was present in many of the MT-projecting neurons within the LPZ. These results demonstrate that V1 lesions trigger neurochemical and structural remodeling of the geniculo-extrastriate pathway, leading to the emergence of nonkoniocellular input to MT. This has potential implications for our understanding of the neurobiological bases of the residual visual abilities that survive V1 lesions, including motion perception and blindsight, and reveals targets for rehabilitation strategies to ameliorate the consequences of cortical blindness.
3.
Bakola S, Burman KJ, Bednarek S, Chan JM, Jermakow N, Worthy KK, Majka P, Rosa MGP.
In: 2021, (Monash University).
Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Afferent Connections of Cytoarchitectural Area 6M and Surrounding Cortex in the Marmoset: Putative Homologues of the Supplementary and Pre-supplementary Motor Areas},
author = {Bakola S, Burman KJ, Bednarek S, Chan JM, Jermakow N, Worthy KK, Majka P, Rosa MGP.},
url = {https://pubmed.ncbi.nlm.nih.gov/34255833/},
year = {2021},
date = {2021-11-23},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
4.
Kwan, Chang, Yu, Mundinano, Fox, Homman-Ludiye, & Bourne
Visual cortical area MT is required for development of the dorsal stream and associated visuomotor behaviors Journal Article
In: 2021, (Monash University).
Abstract | Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Visual cortical area MT is required for development of the dorsal stream and associated visuomotor behaviors},
author = {Kwan, Chang, Yu, Mundinano, Fox, Homman-Ludiye, & Bourne},
url = {https://pubmed.ncbi.nlm.nih.gov/34417331/},
year = {2021},
date = {2021-09-29},
urldate = {2021-09-29},
abstract = {https://pubmed.ncbi.nlm.nih.gov/34417331/
},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
https://pubmed.ncbi.nlm.nih.gov/34417331/
5.
Atapour N, Worthy KM, Rosa MG.
In: 2021, (Monash University).
Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Neurochemical changes in the primate lateral geniculate nucleus following lesions of striate cortex in infancy and adulthood: implications for residual vision and blindsight},
author = {Atapour N, Worthy KM, Rosa MG.},
url = {https://link.springer.com/article/10.1007/s00429-021-02257-0},
year = {2021},
date = {2021-03-20},
urldate = {2021-03-20},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
6.
Quentin Montardy, William C Kwan, Inaki C Mundinano, Dylan M Fox, Liping Wang, Cornelius T Gross, James A Bourne
Mapping the neural circuitry of predator fear in the nonhuman primate Journal Article
In: 2020, (Monash University).
Abstract | Links | BibTeX | Tags: Marmosets, Non-human primates
@article{nokey,
title = {Mapping the neural circuitry of predator fear in the nonhuman primate},
author = {Quentin Montardy, William C Kwan, Inaki C Mundinano, Dylan M Fox, Liping Wang, Cornelius T Gross, James A Bourne},
url = {https://pubmed.ncbi.nlm.nih.gov/33263778/},
doi = {10.1007/s00429-020-02176-6},
year = {2020},
date = {2020-12-02},
urldate = {2020-12-02},
abstract = {In rodents, innate and learned fear of predators depends on the medial hypothalamic defensive system, a conserved brain network that lies downstream of the amygdala and promotes avoidance via projections to the periaqueductal gray. Whether this network is involved in primate fear remains unknown. To address this, we provoked flight responses to a predator (moving snake) in the marmoset monkey under laboratory conditions. We combined c-Fos immunolabeling and anterograde/retrograde tracing to map the functional connectivity of the ventromedial hypothalamus, a core node in the medial hypothalamic defensive system. Our findings demonstrate that the ventromedial hypothalamus is recruited by predator exposure in primates and that anatomical connectivity of the rodent and primate medial hypothalamic defensive system are highly conserved.},
note = {Monash University},
keywords = {Marmosets, Non-human primates},
pubstate = {published},
tppubtype = {article}
}
In rodents, innate and learned fear of predators depends on the medial hypothalamic defensive system, a conserved brain network that lies downstream of the amygdala and promotes avoidance via projections to the periaqueductal gray. Whether this network is involved in primate fear remains unknown. To address this, we provoked flight responses to a predator (moving snake) in the marmoset monkey under laboratory conditions. We combined c-Fos immunolabeling and anterograde/retrograde tracing to map the functional connectivity of the ventromedial hypothalamus, a core node in the medial hypothalamic defensive system. Our findings demonstrate that the ventromedial hypothalamus is recruited by predator exposure in primates and that anatomical connectivity of the rodent and primate medial hypothalamic defensive system are highly conserved.